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IVWM supports the planktonic growth of P. aeruginosa, but not of S. aureus. Planktonic growth curves of Pseudomonas aeruginosa (PAO1) and Staphylococcus aureus (AH133) were performed in Luria-Bertani broth (LB), Fetal Bovine Serum (FBS) and the in vitro wound milieu (IVWM). Optical density (OD600) was measured at intervals of 30 min for 12 h. Error bars represent SEM, n = 3 (biological replicates).
