Figure S1.

Efficacies of transcriptional inhibitors on the suppression of gene and centromeric transcription in HeLa Tet-On, RPE-1, and Drosophila S2 cells. (A) Validation of primers. Serially diluted RNA templates (2×) were subjected to real-time PCR analysis with the indicated primers. Ct values were plotted to template dilution, and R² (R, correlation coefficient) was calculated. Scatter plot with linear regression is shown here. (B) HeLa Tet-On and RPE-1 cells were treated with the indicated conditions (Fig. 1 B), and total RNAs and EU-RNAs were extracted and purified for real-time PCR analysis with the indicated primers. The average and standard error were calculated based on three independent experiments. (C) S2 cells were treated with the indicated conditions, and total RNAs and EU-RNAs were extracted and purified for real-time PCR analysis with the indicated primers. The average and standard error were calculated based on two independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. α-Sat, α-satellite.