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. 2021 Apr 21;218(6):e20201142. doi: 10.1084/jem.20201142

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© 2021 Mittelsteadt et al.

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Figure 1. — Absence of ICOS signaling results in loss of lymphoid T_Rs and altered expression of PI3K targets. (A) Representative flow cytometry plots and quantification of splenic T_Rs (n = 3–6 per group from nine independent experiments). (B) Splenic T_R frequencies at indicated ages (n = 3–5 per group from 16 independent experiments). (C) Representative flow cytometry plots and quantification of splenic cT_Rs (CD44loCD62Lhi) and eT_Rs (CD44hiCD62Llo; n = 3–5 per group from seven independent experiments). (D) Expression of CD62L and CCR7 in splenic T_Rs (CD62L: n = 3–5 per group from five independent experiments; CCR7: n = 3–5 per group from two independent experiments). (E) S6 phosphorylation (p-S6) measured directly ex vivo in splenic T_Rs by flow cytometry (n = 3 or 4 per group from two independent experiments). Mice were age matched within independent experiments, and pooled data are from experiments using mice aged 8–16 wk, except in B, where age is indicated. Statistical significance was determined using one-way ANOVA with Tukey’s post-test. All graphical data are presented as mean values ± SD.