Figure 4.

Differentiation stages of osteoclasts

(A–E) Light Microscopy Images (10×) of:

(A) Myeloid progenitors before differentiation medium is added to the cells.

(B) Early osteoclast differentiation (2 to 4 days after differentiation medium started) indicated with white arrows.

(C) Intermediary osteoclast differentiation, cells are bigger and start to be multinucleated (5 to 7 days after differentiation medium is added).

(D) Full osteoclasts differentiation, cells contain numerous nucleus and are very big (7 to 10 days after differentiation medium added).

(E) Dead Osteoclast (burst), one day after (D).

Star indicates burst osteoclasts. Scale is 400 μm. Arrows indicate multinucleation of the osteoclasts.