Reagent	Final Concentration	Amount in 500 mL Milli-Q water
α-MEM powder	10 g/L	5 g
Sodium bicarbonate (NaHCO3)	2.2 g/L	1.1 g
Penicillin Streptomycin solution	1×	5 mL (from 100× stock)
L-Glutamine	2 mM	5 mL (from 200 mM stock)
MEM amino acids solution	0.375×	3.75 mL (from 50× stock)
Fetal Bovine Serum (FBS) (heat-inactivated)	10%	50 mL
Milli-Q water	-	Up to 500 mL