. 2021 Apr 12;7(4):e06732. doi: 10.1016/j.heliyon.2021.e06732

Table 1.

Ingredients and chemical composition of ingredients and experimental diets.

Feeds (kg)	As fed (kg/sheep/day)	Dry matter (DM; kg/sheep/day)
Corn silage	4.00	1.31
Concentrate	1.20	0.96

Ingredients (g/kg of natural matter)	Concentrates
Ingredients (g/kg of natural matter)	T0	T5	T10
Ground corn	672	672	672
Soybean meal	277	277	277
Calcitic limestone	10.0	10.0	10.0
Sodium bicarbonate	4.0	4.0	4.0
Premix¹	37.0	37.0	37.0
Additive in concentrate (g/kg of natural matter)
Vegetable biocholine²	0.00	4.16	8.33

Chemical composition³	Corn silage	BP	Concentrates¹
Chemical composition³	Corn silage	BP	T0	T5	T10
DM, g/kg	329	964	879	883	885
Ash, g/kg DM	42.1	18.0	65.2	69.3	67.7
CP, g/kg DM	80.1	47.0	156	148	152
NDF, g/kg DM	331	445	106	119	100
ADF, g/kg DM	179	384	45.0	51.0	46.0
EE, g/kg DM	43.9	57.0	34.3	36.7	34.7
TDN, g/kg DM	731	747	821	824	828
TPC (mg GAE 100 g DM)	-	28.2	9.74	9.85	9.89
IC₅₀ (μg/mL)	-	0.30	5.52	5.37	5.11

The premix had: calcium min. 180 max. 220 g; phosphorus min. 32 g; sodium min. 40 g; sulfur min. 20 g; magnesium min. 20 g; cobalt min. 16 mg; iodine min. 17 mg; manganese min. 420 mg; selenium min. 730 mg; zinc min. 730 mg; fluorine max. 600 mg; niacin min. 500 mg; vitamin A min. 95000 IU; vitamin D min. 20000 IU; vitamin E min. 350 IU; monensin sodium 1200 mg; Saccharomyces cerevisiae 2.1 × 10¹⁰ UFC.

Vegetable choline (Biocholine Powder, Nutriquest Technofeed Company, Campinas, SP, Brazil) produced from Azadirachta indica, Citrullus colocynthis, Trachyspermum ammi, Achyranthes aspera plants was used as additive.

CP (crude protein), NDF (neutral detergent fiber), ADF (acid detergent fiber), EE (ether extract), total nutrients digestible (TDN; Calculated as described by Weiss et al., 1992), TPC (total phenolic content) and IC₅₀ (determination of antioxidant activity by elimination of radicals by DPPH).