Figure 5.

Schematic description of the protocol recommended for multi-day training in juvenile zebrafish

Baseline and blank sessions are 60 min long, recall, conditioning and test sessions are 30 min long with no delay in between sessions. During the conditioning session, mildly aversive electric stimuli are delivered at 1.33hz, every time the animal enters the conditioned zone (red). Each day is concluded with a conditioning session to avoid memory extinction overnight. From day two, each protocol starts with a blank session during which the animals are habituated to the arena without any pattern presented. Figure adapted with permission from (Palumbo et al., 2020).