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TUNEL assay for detecting apoptosis after chemogenetic ablation with metrodiniazole
(A–C)Confocal microscopy images of three weeks old Tg(narp:Gal4;UAS-E1b:NTR-mCherry) zebrafish 16 h (A) and 24 h (B) after the start of Metronidazole (MTZ) treatement. Green fluorescent labels TUNEL signal which is a direct measure of cell death (apoptosis). Note that green TUNEL signal is not visible in control animals (C). Scale bar is 50 μm.
