Figure 5.

⁶⁴Cu absorption and accumulation in blood, liver, and spleen were lower in iron-supplemented dams. Shown are intestinal copper (⁶⁴Cu) absorption (A) and ⁶⁴Cu activity in blood (B), liver (C), and spleen (D) of dams fed diets varying in iron concentration for ~3.5 weeks. One-way ANOVA analysis results are as follows: ⁶⁴Cu absorption (A), p = 0.0019; ⁶⁴Cu in blood (B) and liver ⁶⁴Cu (C), p = 0.0004; and spleen ⁶⁴Cu (D), p = 0.0002. When statistically significant differences were noted by one-way ANOVA, Tukey’s post hoc test was utilized to make comparisons between individual groups. Values without common superscript letters are statistically different from one another (p < 0.05) (A–D). All data points are shown, and the mean values are indicated by “+” signs.