Figure 2.

Platelet-Derived Growth Factors-BB (PDGF-BB/PDGF) potentiates ligamentous differentiation in TDCs with small population of MDCs. (a) A total of 4 × 10³ cells (TDCs and/or MDCs, as indicated) were cultured in growth medium for seven days and cell viability was measure using WST assay. A total of 8 × 10⁴ cells/well were seeded in six-well plates, stimulated with vehicle or PDGF-BB for 14 days, and analyzed by (b) Sirius red staining (scale bar = 200 µm) for collagen type 1 and 3, (c) total collagen assay, and (d) qPCR for collagen type 1 and 3 gene expressions. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Data are presented as the mean ± SEM.