Skip to main content
. 2021 Apr 2;13(4):613. doi: 10.3390/v13040613

Figure 5.

Figure 5

Providing truncated S protein in trans failed to rescue L protein secretion but somewhat increased its intracellular level in the context of 0.7 mer construct. N67 (incapable of expressing full-length S protein), N67-nS (incapable of expressing full-length or truncated S protein), or pcDNA3.1 was co-transfected with expression constructs for full-length S protein (N55, N65) or truncated S protein (N65-tS, N65-tS+), or control plasmids (pcDNA3.1, N65-nS) at 1 μg/1 μg ratio. (A,B) Intracellular (A) and extracellular (B) L and S proteins, with GAPDH serving as intracellular control. (C) Extracellular HBsAg. The numbers below anti-preS1 blot in panel A represent the amount of L protein in each lane relative to lane 1 (set at 100) in three independent experiments.