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Fixation with various concentrations of formaldehyde (FA) affects the subcellular distribution of SRPK1. Fluorescent patterns of SRPK1 in HeLa cells fixed for 5 min with 1%, 2%, 3%, or 4% FA. SRPK1 was detected using the anti-SRPK1 monoclonal antibody, while nuclei were stained with PI. Scale bar: 10 µM.
