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. 2021 Mar 30;10(4):759. doi: 10.3390/cells10040759

Figure 9.

Figure 9

ATR/ATM inhibition significantly prevented the nuclear translocation of SRPK1 in 5-FU-treated HeLa and T24 cells. Fluorescent patterns of SRPK1 in HeLa (left panel) and T24 cells (right panel) treated either alone with 5-FU (20 µg/mL in HeLa, 5 µg/mL in T24) for 48 h, or co-treated for 48 h with 5-FU plus 10 µM KU-55,933, 5-FU plus 10 µM VE-821, or 5-FU plus 10 µM KU-55,933 plus 10 µM VE-821. SRPK1 was detected using the anti-SRPK1 monoclonal antibody, while nuclei were stained with PI. Scale bar: 10 µM.