Table 2.

Design of the panel for multiplex fluorescent immunohistochemistry (fIHC) staining.

#	AR	Antibody	Dilution	Incubation	Secondary	OPAL	Dilution
1	6	CD68	1:200	1 h/RT	Ms + Rb Opal HRP polymer	540	1:200
2	6	CD163	1:100	OVN/4 °C		620	1:100
3	9	ARG1	1:200	1 h/RT		650	1:200
4	9	CD80	1:50	OVN/4 °C		520	1:50
5	6	Cytokeratin	1:800	1 h/RT		690	1:200
6	6	DAPI	1:15	5 min/RT	–	–	–

AR—antigen retrieval buffer; RT—room temperature; OVN—overnight.