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. 2021 Mar 25;10(4):263. doi: 10.3390/biology10040263

Table 2.

MALDI-TOF mass fingerprinting data of the nine differentially expressed proteins isolated from Metarhizium anisopliae infecting Coptotermes curvignathus. Proteome changes in the infecting fungus were compared in a time-line post-inoculation (PI) experiment to control proteins, extracted from 2D electrophoresis gels prior to identification.

Spot ID Score No. of Peptides Matched Sequence Coverage (%) Theoretical Mr/pI Protein Identity Metabolic Pathway Cellular Location Accession no. (NCBInr), (Organism)
SWISS PROT NCBInr Mr (kDa) pI
16 46 174 6 57 29.14 5.64 Methylthioribulose-1-phosphate dehydratase L-methionine biosynthesis via
salvage pathway
Cytoplasm OAA48651 (Metarhizium rileyi RCEF 4871)
27 90 196 28 34 119.45 5.63 Sec8 exocyst complex component specific domain protein Exocystosis Exocyst KID86834 (Metarhizium guizhouense ARSEF 977)
29 55 447 13 47 45.92 9.19 Lipoic acid
synthetase
Lipoate
biosynthetic process
Mitochondrion XP_014580459 (Metarhizium majus ARSEF 297)
32 65 914 17 31 87.74 6.29 Heat shock protein 78 ATP-binding Nuclear region XP_014546332 (Metarhizium brunneum ARSEF 3297)
51 48 414 15 39 60.05 7.25 ATP-dependent RNA helicase DBP8 ATP-binding Nuclear region/cytoplasm OAA38096 (Metarhizium rileyi RCEF 4871)
87 48 434 13 36 53.00 6.06 ATP dependent RNA helicase DBP5 ATP-binding Nuclear region/cytoplasm XP_007808948 (Metarhizium acridum CQMa 102)
88 45 334 7 30 41.82 6.12 Methylthioribose-1-phosphate isomerase L-methionine
biosynthetic process
Nuclear region/cell projection XP_014549852 (Metarhizium brunneum ARSEF 3297)
89 39 43.5 8 29 58.41 5.43 Defects-in-morphology protein 1-like Nucleic acid
phosphodiester bond hydrolysis
Nuclear region/cytosol OAA48471 (Metarhizium rileyi RCEF 4871)
90 62 21.8 22 20 56.04 6.4 Peroxisomal catalase Peroxidase/
oxidoreductase activity
Peroxisome XP_007809457 (Metarhizium acridum CQMa 102)