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. 2021 Mar 30;12(4):304. doi: 10.3390/insects12040304

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Log transformed difference between ZIKV and CHIKV detected in the bloodmeals and saliva of individual infected mosquitoes after they were given different blood feeding treatments. (A) Infectious ZIKV mean difference: None = 2.21, Artificial feeder = 3.65, Artificial feeder + mixing = 3.69. (B) ZIKV genome copies mean difference: none = 5.05, artificial feeder = 7.06, artificial feeder + mixing = 6.35. (C) Infectious CHIKV mean difference: none = 2.06, artificial feeder = 3.41, artificial feeder + mixing = 3.73. (D) CHIKV genome copies mean difference: none = 5.19, artificial feeder = 6.82, artificial feeder + mixing = 6.65. Graphs show both PFUs determined by plaque assay and RNA determined by qRT-PCR. Titer LOD = 2 PFU; genome copy LOD = 10 genomes. Means are horizontal bars (one-way ANOVA and post hoc Tukey test; * p ≤ 0.05).