Table 4.

Detection of ZIKV from forced salivations after infected mosquitoes blood fed on immunocompromised mice.

Mouse ID	Number of Mosquitoes That Blood Fed	Pooled Saliva-Titer (PFU)	Pooled Saliva-Genome Copies	Mouse Became Infected as Determine by Viral RNA Detection in Tissue/Blood?
080	4	0	2.8 × 105	Yes
086	2	0	2.0 × 104	Yes
095	1	0	7.2 × 102	Yes
109	2	0	2.0 × 104	Yes
165	3	0	1.3 × 103	Yes
166	1	0	5.0 × 103	Yes
169	2	0	6.8 × 104	Yes
174	3	0	3.3 × 103	Yes

Pooled saliva titers were determined by plaque assay, limit of detection (LOD) = 2 PFU. Pooled saliva genome copies were determined by qRT-PCR, LOD = 10 genome copies. In total, 53 mice became infected after being bitten by ZIKV-infected mosquitoes. Eight of these mice (shown above) became infected even though the mosquitoes that bit them had undetectable titers of virus in their saliva as measured by FS post-blood feeding.