Table 6.
Detection of CHIKV from forced salivations and from the blood remaining in the artificial feeders after infected mosquitoes blood fed on them.
| Group Number | Number of Mosquitoes That Blood Fed | Pooled Saliva-Titer (PFU) | Pooled Saliva-Genome Copies | Remaining Blood in the Artificial Feeder-Titer (PFU) | Remaining Blood in the Artificial Feeder-Genome Copies |
|---|---|---|---|---|---|
| 1 | 2 | 2.1 × 102 | 1.1 × 105 | 0 | 0 |
| 2 | 11 | 3.7 × 103 | 1.8 × 106 | 0 | 0 |
| 3 | 10 | 2.0 × 102 | 9.5 × 104 | 0 | 0 |
| 4 | 7 | 3.3 × 102 | 5.0 × 104 | 0 | 2.8 × 103 |
| 5 | 6 | 2.1 × 102 | 1.0 × 105 | 0 | 0 |
Pooled saliva titers determined by plaque assay, limit of detection (LOD) = 2 PFU. Pooled saliva genome copies determined by qRT-PCR LOD = 10 genome copies.