Table 3.
Fluorocromes | Tube 1: Lymphocytes B | Tube 2: Lymphocytes T | ||
---|---|---|---|---|
Antibody (Clon) | Manufacter | Antibody (Clon) | Manufacter | |
FITC | CD19 (HIB19) | BD Biosciences | CD19 (HIB19) CD4 (RPA-T4) |
BD Biosciences |
PE | IgM (G20-127) | BD Biosciences | CD25 (2A3) | BD Biosciences |
PE-Cy7 | CD27 (M-T271) | BD Biosciences | CD45RO (UCHL1) | BD Biosciences |
APC | CD38 (HB7) | BD Biosciences | - | BD Biosciences |
APC-Cy7 | CD24 (ML5) | BD Biosciences | HLA-DR (L243) | BD Biosciences |
PerCP | CD45 (HI30) | BioLegend | CD8 (SK1) | BD Biosciences |
V450 | IgD (IA6-2) | BD Biosciences | CD127 (BV421) | BD Biosciences |
V500 | CD3 (UCHT1) | BD Biosciences | CD3 (UCHT1) | BD Biosciences |