Figure 3.

Viral titers expectorated with each successful transmission event by Rockefeller individuals. Only mosquitoes which had successful transmission over time are shown. A successful transmission event is defined by a positive serum sample via qRT-PCR and growth on Vero cells. (a) Each shaded square represents a transmission event, with white squares representing no detectable virus. Other squares are scored from lowest viral titer (light blue) to highest viral titer (navy). (b) Viral titers present in serum were compared for blood fed (F) vs. probed (P). Viral titers range from lowest detectable titer (light blue) to highest detectable titer (navy). Asterisks (*) indicate where recovered quantity is below the limit of detection from our sensitivity analysis and reflect qRT-PCR values of genome equivalents (viral RNA copies/100 μL) rather than pfu/100 μL.