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. 2021 Mar 30;12(4):507. doi: 10.3390/genes12040507

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Overview of the DEEPGEN^TM assay workflow. Blood samples from liquid biopsies or reference materials are used to isolate cell-free DNA (cfDNA). Unique molecular identifiers (UMI) are ligated to individual cfDNA strands, followed by an enrichment of cancer-relevant sequences using a customized primer panel. The enriched NGS libraries are subsequently sequenced on a Illumina NovaSeq 6000 and analyzed with the DEEPGEN^TM bioinformatics pipeline. The output is a mutation table that contains the variant frequency for all targeted locations.