Figure 4.

Reproducibility of DEEPGEN^TM assay. Results of six independent replicates of a 0.5% Seraseq^TM ctDNA Mutation Mix v2 reference standard were compared across 20 validated target variants. Details for each validated variant are listed in Table S3. (A) Heatmap representation of the six replicates with their measured variant frequencies. VAF around the expected values are shown in blue, values outside of the acceptable range (expected VAF ± 50%) in white. (B) Overview of all 20 validated targets (at 0.5% VAF) with their measured frequencies. The permissible range (0.25%–0. 75%) of the measured values is shown as a grey background. Box plots show the interquartile range (IQR) with whiskers representing the range of the 5–95 percent confidence interval.