. 2021 Mar 30;12(4):507. doi: 10.3390/genes12040507

Table 1.

Summarized performance metrics of the DEEPGEN^TM assay. The sensitivity, specificity, PPV, NPV and accuracy of the variant calling was calculated for the verified VAF of 0%, 0.125%, 0.25% and 0.5%. The overall result (across validated VAFs) and the 95% confidence intervals (CI) are displayed.

Performance Metrics	Formula	VAF Specific Results		Overall Results	Overall 95% CI
Sensitivity	$S e n s i t i v i t y = (\frac{T r u e P o s i t i v e s}{T r u e P o s i t i v e s + F a l s e N e g a t i v e s}) \times 100$	0.5% 0.25% 0.125%	98.3% 98.3% 76.7%	91.1%	86.0%–94.8%
Specificity	$S p e c i f i c i t y = (\frac{T r u e N e g a t i v e s}{T r u e N e g a t i v e s + F a l s e P o s i t i v e s}) \times 100$	0%	95%	95.0%	86.1%–99.0%
PPV	$P P V = (\frac{T r u e P o s i t i v e s}{T r u e P o s i t i v e s + F a l s e P o s i t i v e s}) \times 100$	0.5% 0.25% 0.125%	95.2% 95.2% 93.9%	98.2%	94.8%–99.6%
NPV	$N P V = (\frac{T r u e N e g a t i v e s}{T r u e N e g a t i v e s + F a l s e N e g a t i v e s}) \times 100$	0.5% 0.25% 0.125%	98.3% 98.3% 80.3%	78.1%	66.8%–86.9%
Accuracy	$A c c u r a c y = (\frac{T r u e P o s i t i v e s + T r u e N e g a t i v e s}{T o t a l T a r g e t s E x a m i n a t e d}) \times 100$	0.5% 0.25% 0.125%	96.7% 96.7% 85.8%	92.1%	87.9%–95.2%