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. 2021 Apr 2;10(4):555. doi: 10.3390/antiox10040555

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression of nitric oxide synthase (NOS) isoforms. Western blot analysis for the expression of inducible (iNOS) and endothelial (eNOS) NOS in the rat heart after ischemia–reperfusion. The panels show the levels of the NOS isoforms iNOS and eNOS in cardiac homogenates of rat hearts submitted to the ischemia–reperfusion protocol in the absence or presence of the GSNOR inhibitor C2 (1 µmol/L). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as load control. Densitometric units of each corresponding isoform were normalized by the levels of GAPDH in each heart. n = 3 in each group.