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. 2021 Apr 4;10(4):805. doi: 10.3390/cells10040805

Figure 4.

Figure 4

Endothelin-1 induced hypertrophy reduced autophagy in neonatal cardiomyocytes. (A) Immunoblot analysis of p62 and (B) LC3-I and (C) LC3-II. (D) Analysis of LC3-II/GAPDH to LC3-I/GAPDH. (E) Representative immunoblots of LC3-I, LC3-II, p62 and their respective GAPDH control. Data are presented as fold change (Mean + SEM) of biological replicates and statistical analyses were performed by unpaired t-test (indicated with #) or one-sample t-test (indicated with a), comparing two treatments directly. Statistically significant differences are shown by p < 0.05, * vs. control and † vs. ET-1.