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. 2021 Mar 27;9(4):693. doi: 10.3390/microorganisms9040693

Figure 2.

Figure 2

Adherence patterns of representative hybrid uropathogenic Escherichia coli (UPEC) strains. The adherence patterns were assessed as preconized in HeLa cells in assays with an incubation period of 3 h or 6 h, at 37 °C in the presence of 2% D-mannose, using a multiplicity of infection of 10. The preparations were stained with May-Grünwald/Giemsa and observed under a light optical microscope (1000× magnification). All hybrid UPEC strains were adherent, and different adherence patterns were identified. Representative hybrid UPEC/EAEC (enteroaggregative E. coli) strains (3 h) are in panels (A,B), and a hybrid UPEC/aEPEC (atypical enteropathogenic E. coli) strain (6 h) in panel (C). (A). HSP 117, displaying a non-characteristic aggregative adherence pattern with small loose clusters and spread foci of adherent bacteria; (B). HSP 414, showing the typical aggregative adherence pattern; (C). HSP 278, showing the localized adherence-like pattern. (D). E. coli 042 (EAEC—aggregative adherence pattern control); (E). E. coli strain E2348/69 (typical EPEC—localized adherence pattern control); (F). E. coli strain 4581-2 (aEPEC—localized adherence-like pattern control); (G). E. coli strain CFT073 (UPEC control); (H). E. coli strain HB101 (K-12 derived laboratory strain, non-adherent control); (I). Non-infected control cells.