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. 2021 Apr 3;10(4):295. doi: 10.3390/biology10040295

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Alkaline phosphatase (ALP) expression in DBSCs. (a) ALP expression assayed by ALP cytochemical assay (purple staining) in DBSCs cultured in osteogenic conditions and treated with irisin or not (Ctr) for 7 days. Representative phase contrast pictures were chosen for the figure. Scale bar: 100 μm. (b) The graph shows the quantification of ALP % area presented as box-and-whisker plots with median and interquartile ranges, from max to min, and is representative for five independent experiments performed in quadruplicates.