Figure 1.

Gene structure of slr0320 and growth of wild type (WT), Δslr0320 and PpetJ::slr0320 strains. (a) Organization of slr0320 in Synechocystis. The insertion position of a transposon including a chloramphenicol-resistant cassette (Cm^R, green) flanked by two insertion sequences (Tn, orange) is indicated; two conserved domains of slr0320 were indicated under the slr0320 coding region (red); (b) growth of WT, Δslr0320 and PpetJ::slr0320 strains on agar plates under normal light (NL) and high light (HL). The cells grown under NL were harvested and resuspended in fresh BG-11 medium. Then, they were diluted to 0.5, 0.05, 0.005 at OD750 and 10 μL liquid cells were placed on the agar plate. The pictures were taken after 6 days; (c) growth curves of WT, Δslr0320 and PpetJ::slr0320 strains in liquid medium under NL and HL. The initial OD750 was 0.05. Student’s t test was applied. Red asterisks mark significant differences for the Δslr0320 mutant vs. WT and PpetJ::slr0320 strains, with a significance level of p < 0.05. The results are plotted as the means from three independent experiments ± SD.