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. 2021 Mar 26;10(4):266. doi: 10.3390/biology10040266

Figure 2.

Figure 2

PER2 expression oscillates in human eSCC cells in spite of alterations in the pattern of clock-related gene expression. (A) Experimental design. (B) Histogram depicting the expression of clock related genes measured by RNA sequencing in the KYSE-140 human eSCC cell line. Data are represented as the mean of a biological duplicate. FDR is indicated for each gene. Benjamini–Hochberg method on the p-values was used to control the false discovery rate (FDR). (C) Same as in (B) in the KYSE-180 human eSCC cell line. (D) Same as in (B) in the KYSE-270 human eSCC cell line. (E) Same as in (B) in the KYSE-410 human eSCC cell line. (F) Same as in (B) in the KYSE-450 human eSCC cell line. (G) Co-immunostaining of p63 and Krt14 in the KYSE-410 human eSCC cell line. (H) Experimental design to monitor the activity of the PER2 promotor in KYSE-410 cells. (I) Real time monitoring of luciferase activity in the KYSE-410 human eSCC cell line. FDR>5% are considered non-significant (N.S).