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. 2021 Mar 26;10(4):266. doi: 10.3390/biology10040266

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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PER2 expression oscillates in primary culture of mouse eSCC cells and influences sensitivity to chemotherapy. (A) Genetic strategy and experimental design. (B) Representative picture of 4-NQO-induced eSCC in K5:p53:YFP mouse esophagus. (C) Co-immunostaining of p63 and Krt14 in normal esophagus and 4-NQO-induced eSCC. (D) FACS plot showing the expression of EpCam and lineage negative markers (CD45, CD31, and CD140a) in control esophageal cells, and YFP and lineage negative markers in 4-NQO-induced eSCC. (E) Expression of clock-related genes measured by RNA sequencing in FACS sorted Epcam+ normal esophagus epithelial cells (n = 2) and YFP+ 4-NQO-induced eSCC epithelial cells (n = 3). FDR is indicated for each gene. Benjamini–Hochberg method on the p-values was used to control the false discovery rate (FDR). FDR>5% are considered non-significant (N.S). “Epith” means epithelial cells.