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. 2021 Mar 27;10(4):742. doi: 10.3390/cells10040742

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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High glucose treatment shifted endothelial cell angiomiR expression towards an anti-angiogenic phenotype. (A) Primary miR and (B) mature miR levels relative to HPRT housekeeping gene and exogenous spike-in cel-miR-39-3p, respectively. Pro-angiogenic pri-miRs and miR are underlined in red, while angiostatic pri-miRs and miRs are underlined in blue. Data are means ± SEM (n = 6). Two-way ANOVA showed no effect of glucose concentration on the expression of pri-miR-126, pri-miR-17-92, and pri-miR-15; *** indicates significative difference compared to 5 mM of glucose (p < 0.001)). (C,D) VEGF-A and THBS-1 mRNA relative to HPRT in HDMEC treated with 5 or 30 mM glucose concentrations for 24 h (data are means ± SEM, n = 9). (E,F) Immunoblots of miR targets VEGF-A and THBS-1 (A6.1) after 24 h treatment under 5 or 30 mM glucose conditions. α,β-TUBULIN was used as a loading control. Data are means ± SEM; * indicates significative differences (p < 0.05, t-test, n = 5-10).

High glucose treatment shifted endothelial cell angiomiR expression towards an anti-angiogenic phenotype. (A) Primary miR and (B) mature miR levels relative to HPRT housekeeping gene and exogenous spike-in cel-miR-39-3p, respectively. Pro-angiogenic pri-miRs and miR are underlined in red, while angiostatic pri-miRs and miRs are underlined in blue. Data are means ± SEM (n = 6). Two-way ANOVA showed no effect of glucose concentration on the expression of pri-miR-126, pri-miR-17-92, and pri-miR-15; *** indicates significative difference compared to 5 mM of glucose (p < 0.001)). (C,D) VEGF-A and THBS-1 mRNA relative to HPRT in HDMEC treated with 5 or 30 mM glucose concentrations for 24 h (data are means ± SEM, n = 9). (E,F) Immunoblots of miR targets VEGF-A and THBS-1 (A6.1) after 24 h treatment under 5 or 30 mM glucose conditions. α,β-TUBULIN was used as a loading control. Data are means ± SEM; * indicates significative differences (p < 0.05, t-test, n = 5-10).