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. 2021 Mar 27;10(4):742. doi: 10.3390/cells10040742

Figure 5.

Figure 5

High glucose conditions decrease DROSHA protein expression in mouse skeletal muscle tissue. (A) Immunoblot of DROSHA expression in mouse primary endothelial cells isolated from skeletal muscle tissue (mSMECs) following 24 H treatment with high (30 mM) or low (5 mM) glucose concentrations. α/β-TUBULIN was used as a loading control. High glucose concentration (30 mM) decreased DROSHA protein expression (data are means ± SEM, n = 6, * p < 0.05). (B) Immunoblot of DROSHA expression in gastrocnemius muscles from wild-type (wt/wt) and leptin receptor deficient (db/db) mice (data are means ± SEM, n = 4–7, p = 0.034). The db/db mice expressed significantly less DROSHA protein compared to their wild-type littermates (data are means ± SEM, n = 4–7, * p < 0.05). (C) Correlation analysis between DROSHA protein expression and resting blood glucose levels in gastrocnemius muscles from 4-week-old mice, including 2 wt/wt, 2 db/db and 9 db/wt mice. Resting blood glucose is negatively correlated with DROSHA protein expression in mice skeletal muscle (n = 13, ** p = 0.006).