Table 1.
List of Taqman probes used to measure RNA transcripts. For pri-miR and miR target name, the first three letters signify the organism: ‘hsa’ for human origin, ‘cel’ for C. elegans and ‘mmu’ for murine origin. Then, the three following letters ‘mir’ and ‘miR’ indicate sequences of pri-miR and mature miR, respectively. At the end of the target name, 3p and -5p indicate that the sequence originates from 3′ or 5′ arm, respectively.
| Assay ID | Target | Sequences |
|---|---|---|
| Hs03303230_pri | hsa-mir-126 | CGCUGGCGACGGGACAUUAUUACUUUUGGUACGC GCUGUGACACUUCAAACUCGUACCGUGAGUAAUA AUGCGCCGUCCACGGCA |
| Hs03302582_pri | hsa-mir-15a | CCUUGGAGUAAAGUAGCAGCACAUAAUGGUUUGU GGAUUUUGAAAAGGUGCAGGCCAUAUUGUGCUGC CUCAAAAAUACAAGG |
| Hs03295901_pri | hsa-mir-17 | GUCAGAAUAAUGUCAAAGUGCUUACAGUGCAGGU AGUGAUAUGUGCAUCUACUGCAGUGAAGGCACUU GUAGCAUUAUGGUGAC |
| 477887_mir | hsa-miR-126-3p | UCGUACCGUGAGUAAUAAUGCG |
| 477888_mir | hsa-miR-126-5p | CAUUAUUACUUUUGGUACGCG |
| 477858_mir | hsa-miR-15a-5p | UAGCAGCACAUAAUGGUUUGUG |
| 478551_mir | hsa-miR-18a-5p | UAAGGUGCAUCUAGUGCAGAUAG |
| 478293_mir | cel-miR-39-3p | UCACCGGGUGUAAAUCAGCUUG |
| 482962_mir | mmu-miR-15a-5p | UAGCAGCACAUAAUGGUUUGUG |
| Hs02800695_m1 | HPRT | GGACTAATTATGGACAGGACTGAAC |
| Hs01066930_m1 | MDM2 | GAACAAGAGACCCTGGTTAGACCAA |
| Hs00900055_m1 | VEGF-A | ACATCACCATGCAGATTATGCGGAT |
| Hs00962908_m1 | THBS-1 | ACACAATCCGGATCAGCTGGACTCT |
| Hs00229023_m1 | DICER1 | GTGCTACCCAAAAGCAATTCCAGAG |
| Hs00203008_m1 | DROSHA | AATGATCCGGACCTGCGCGAAGTCT |