Table 1.
Primers used for reverse transcribed-quantitative polymerase chain reaction (RT-qPCR). Transforming growth Figure 4. nuclear factor erythroid 2-related factor 2 (Nrf2); monocyte chemotactic protein (Mcp1); fibronectin (Fn); collagen type I and III (Col I, Col III); alpha smooth muscle actin (α-Sma); catalase (Cat); glutathione peroxidase (Gpx); manganese-dependent superoxide dismutase (MnSOD); copper/zinc superoxide dismutase (Cu/ZnSOD).
| Mouse Gene | Sequence | |
|---|---|---|
| Tgf-β | Forward Reverse |
CCTGAGTGGCTGTCTTTTGA CGTGGAGTTTGTTATCTTTGCTG |
| Tnf-α | Forward Reverse |
TCCCAGGTTCTCTTCAAGGGA ACAAGGTACAACCCATCGGC |
| Nf-κB | Forward Reverse |
GTGATGGGCCTTCACACACA CATTTGAACACTGCTTTGACT |
| F4/80 | Forward Reverse |
ACCACAATACCTACATGCACC AAGCAGGCGAGGAAAAGATAG |
| Nrf2 | Forward Reverse |
TCCCATTTGTAGATGACCATGAG CCATGTCCTGCTCTATGCTG |
| Mcp1 | Forward Reverse |
GCAGTTAACGCCCCACTCA CCAGCCTACTCATTGGGATCA |
| Fn | Forward Reverse |
CTTTGGCAGTGGTCATTTCAG ATTCTCCCTTTCCATTCCCG |
| Col I | Forward Reverse |
CATAAAGGGTCATCGTGGCT TTGAGTCCGTCTTTGCCAG |
| Col III | Forward Reverse |
GAAGTCTCTGAAGCTGATGGG TTGCCTTGCGTGTTTGATATTC |
| α-Sma | Forward Reverse |
GTGAAGAGGAAGACAGCACAG GCCCATTCCAACCATTACTCC |
| Gapdh | Forward Reverse |
CTTTGTCAAGCTCATTTCCTGG TCTTGCTCAGTGTCCTTGC |
| Cat | Forward Reverse |
CCTCCTCGTTCAGGATGTGGTT CGAGGGTCACGAACTGTGTCAG |
| Gpx | Forward Reverse |
TGCTCATTGAGAATGTCGCGTCTC AGGCATTCCGCAGGAAGGTAAAGA |
| MnSod | Forward Reverse |
ATGTTGTGTCGGGCGGCG AGGTAGTAAGCGTGCTCCCACACG |
| Cu/ZnSod | Forward Reverse |
GCAGGGAACCATCCACTT TACAACCTCTGGACCCGT |