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. 2021 Mar 31;13(4):597. doi: 10.3390/v13040597

Figure 6.

Figure 6

Figure 6

Export inhibition by wheat germ agglutinin (WGA) treatment does not interfere with capsid docking at the nuclear envelope. U2OS cells were infected with WT KSHV virions. After 30 min, cells were incubated for 5 min with 5 µg/mL Cy5-WGA (cyan) along with 30 µg/mL digitonin (DGt). Cells were then washed and further incubated for 4.5 h, and then treated with Triton X-100 to remove the cytoplasm. Capsids and Nup214 were detected as described in Figure 3 (A). The relative localization of the capsids and Nup214 was detected by STED microscopy, as described in Figure 4. Scale bars = 7.5 µm (B). 150 U2OS nuclei and 1630 capsids treated with Cy5-WGA and digitonin, and 108 U2OS nuclei and 1239 capsids treated with digitonin-only as a control, were inspected to determine the number of capsids associated with the nuclear envelope. The average number of capsids on the nuclear envelope was similar and no significant difference between WGA and control were found using Mann–Whitney U-test. p = 0.2115. Dots represent extreme values (C).