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. 2021 Apr 2;13(4):609. doi: 10.3390/v13040609

Figure 5.

Figure 5

Glycyrrhizin potently inhibits the viral main protease (Mpro). The inhibition of SARS-CoV-2 Mpro by glycyrrhizin was measured using the “Fluorogenic 3CL Protease, MBP-tagged (SARS-CoV-2) Assay Kit”. Different glycyrrhizin concentrations (30 µM and 2000 µM) and the complete protease inhibitor GC376 (100 µM) were dissolved with 90 ng Mpro in 30 µL 0.5 M DTT Buffer and incubated for 30 min at room temperature. Subsequently, the 3CL Protease (SARS-CoV-1/SARS-CoV-2) substrate was added and the protease activity was measured after overnight incubation at a wavelength of 360 nm/460nm (exc/em). The experiment was performed in triplicates. Measurement was performed using Mithras LB 943 after 12 h incubation. Statistical analysis was undertaken with a non-parametric ANOVA test. Comparisons were considered significant at ** p < 0.01 and n.s. = no significance. All experiments were performed in biological replicates. Error bars represent the standard derivation of the mean (SD).