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. 2021 Mar 26;10(4):731. doi: 10.3390/cells10040731

Figure 2.

Figure 2

Beclin-1 expression in sensitive and tolerant macrophages. Stearic acid (free fatty acid: FFA)-exposed sensitive and tolerant RAW264.7 cell macrophages (RAW) were stimulated with or without lipopolysaccharide (LPS) for 4 h or 24 h. Loading control: GAPDH. (A) Beclin-1 protein expression was detected in the cytosolic extract by Western blot. (B) Western blot image quantification using image-J software (n = 5 blots; * p < 0.05). (C) FFA-exposed tolerant RAW cells were treated with SIRT2 inhibitor AK-7 (25 µM) or vehicle (DMSO; equal volume) and incubated further for 20 h and stimulated with or without LPS as indicated. Beclin-1 protein expression was detected in the cytosolic extract by Western blot (D). Western blot image quantification of beclin-1using image-J software (n = 5 blots; * p < 0.05).