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. 2021 Mar 26;10(4):731. doi: 10.3390/cells10040731

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SIRT2 interacts with p62, LC3b and α-tubulin in sensitive and tolerant macrophages. Stearic acid (free fatty acid: FFA)-exposed sensitive and tolerant RAW264.7 cell macrophages (RAW) were stimulated with or without LPS as indicated. FFA-exposed tolerant RAW cells were treated with AK-7 or vehicle (DMSO) and stimulated with or without LPS as indicated. (A) SIRT2 was immunoprecipitated (IP) from whole-cell lysates of FFA-exposed sensitive and tolerant RAW cells using an anti-SIRT2 antibody followed by immunoblot (IB) analysis of the indicated proteins including LC3b-II, p62, total alpha-tubulin and SIRT2. Pull down with IgG control antibody was used as a negative control. (B) Western blot analysis of LC3b, p62, total alpha-tubulin and SIRT2 in the whole cell lysate used as input for the SIRT2 IP. Loading control: GAPDH.