Skip to main content
. 2021 Apr 2;10(4):789. doi: 10.3390/cells10040789

Figure 5.

Figure 5

Altered markers of inflammation and complement components in AMD and high-risk iPSC-RPE. (A,B) Gene expression analysis of complement-related genes in iPSC-RPE from (A) No AMD (5 donors, 8 lines) and AMD (13 donors, 20 lines) donors or (B) CFH low-risk (No AMD n = 3 donors, 5 lines; AMD n = 5 donors, 8 lines) and high-risk (No AMD n = 1 donor, 2 lines; AMD n = 8 donors, 12 lines). Results shown are fold-change in expression of (A) AMD relative to the average of No AMD samples (dashed line) or (B) high-risk relative to the average of low-risk donors (dashed line). ELISA for IL-6 (C), CFH (D), and C3a (E) in media from iPSC-RPE comparing No AMD (7 donors, 10 lines) and AMD (12 donors, 17 lines) donors or low risk (No AMD 4 donors, 6 lines; AMD 4 donors, 5 lines) and high risk (No AMD 3 donors, 4 lines; AMD 9 donors, 13 lines) donors. NA = No AMD. All data are mean ± SEM. ** p < 0.01, * p < 0.05, # p < 0.1 determined by un-paired t-tests. All other comparisons were not statistically different.