Table 1.
Cytotoxicity and anti-bovine viral diarrhea virus (BVDV) activity of Salvia officinalis essential oil and their main constituents.
| Compounds | MDBK | BVDV |
|---|---|---|
| a CC50 | b EC50 | |
| * Salvia officinalis | > 100 | 50 ± 3 |
| Camphor | > 100 | > 100 |
| Camphene | > 100 | > 100 |
| 2+β Thujone | > 100 | > 100 |
| Limonene | > 100 | > 100 |
| β-Pinene | > 100 | > 100 |
| Trans-Caryophyllene | > 100 | 90 ± 1 |
| α-Humulene | > 100 | 36 ± 0.5 |
| 1,8-Cineole | > 100 | > 100 |
| Reference Compound | ||
| 2′-C-Me-Guanosine | > 100 | 1.6 ± 0.2 |
a Compound concentration (μM) required to reduce the viability of mock-infected Madin-Darby bovine kidney (MDBK) cells by 50%, as determined by the MTT method. b Compound concentration (μM) required to achieve 50% protection of MDBK cells from the BVDV-induced cytopathogeneticy, as determined by the MTT method. * (µg/mL). Data are mean values + standard deviation (SD) for three independent determinations. When SD is not shown, the variation among samples results in less than 15%. CC50 (50% cytotoxic concentration), EC50 (50% effective concentration).