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. 2021 Apr 5;13(4):495. doi: 10.3390/pharmaceutics13040495

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Isolation and characterisation of adipose tissue-derived stem cell extracellular vesicles (ADSC-EVs). (A) A schematic diagram demonstrating the isolation of EVs from ADSCs (created with BioRender.com). (B) Western blot analysis of ADSCs (cells and EVs); anti-CD81, anti-GM130, calnexin, and anti-cytochrome-C antibodies were used. (C) The morphology of ADSC-EVs visualised by TEM (scale bar: 200 nm). (D,E) NTA of ADSC-EVs (n = 5). The results are expressed as the mean ± SD values of the experiment.