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. 2021 Apr 1;10(4):786. doi: 10.3390/cells10040786

Figure 2.

Figure 2

The effect of MALAT1 knockdown on the OA osteoblast transcriptome. (A) Knockdown of MALAT1 expression in primary OA osteoblasts. Osteoblasts were transfected with either a non-targeting control LNA (NC) or one of two LNAs targeting MALAT1, M1(1) and M1(2). Expression of MALAT1 was determined by qPCR 24 h after transfection and normalised to 18S. (B) Heatmap of differentially expressed genes (>1.5-fold, p < 0.05) as determined by RNAseq (Quantseq) following 24 h LNA-mediated MALAT1 knockdown. (C) Table of the most upregulated and down-regulated genes following MALAT1 knockdown, with Venn diagram illustrating the total numbers of genes differentially expressed (>1.5-fold, p < 0.05) with each of the MALAT1 LNAs. Values represent the mean fold change between control LNA and MALAT1(1) LNA and between control LNA and MALAT1(2) LNA. (D) Top canonical pathways and cellular processes as determined by Ingenuity Pathway Analysis of the differentially expressed genes (fold change > 1.5, p < 0.05) following MALAT1 knockdown, with logP values representing the significance between the pathway/process and the gene dataset and n representing the numbers of genes within the dataset aligned to the pathway/process.