Figure 6.

Networks of functional protein–protein interactions of the top 20 downregulated proteins in response to PE5. (A,B) The functional protein–protein interactions of the top 20 downregulated proteins, and the significant nodes of each network were identified and rebuilt as a network of the signaling pathway in cancer using STITCH database version 5.0 (http://stitch.embl.de/, access date: 24 June 2020). (C,D) The key proteins p-PI3K, PI3K, mTOR, p-mTOR, AKT, p-AKT, Bcl-2, and Bax were determined by western blotting in H460 cells, and the immunoblot signal intensities were quantified by densitometry. Data represent the mean ± SD (n = 3), (* p < 0.05, ** p < 0.01, compared with the untreated control).