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. 2021 Mar 30;10(4):659. doi: 10.3390/plants10040659

Figure 2.

Figure 2

Effect of AYC-CS-E treatment on cell viability of non- and ultraviolet B (UVB)-irradiated human keratinocytes (HaCaT cells). (a) The HaCaT cell line at a concentration of 1 × 105 cells/well was seeded in a 48-well plate, incubated for 12 h in an incubator at 37 °C and 5% CO2, and treated with the indicated concentrations (2, 8, 16, or 32 μL/mL) of AYC-CS-E (30 mg/mL) for 24 h. The cell viability was measured using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Con: non-treated HaCaT cells, (b) The cells (1 × 105 cells/well in 48-well plate) were treated with various concentrations (0.125 to 64 μL/mL) of AYC-CS-E for 24 h after being exposed to 8 mJ/cm2 UVB irradiation, as described in the Materials and Methods section. The cell viability for each condition was measured using the MTT assay. All bar graphs show the means ± standard deviation (SD) of 3 samples. One representative plot out of three independent experiments is shown. ** p < 0.01, and *** p < 0.001.