Figure 2.

Antiviral activity of salinomycin on porcine epidemic diarrhea virus (PEDV) infection in Vero cells. Vero cells mock pretreated with DMSO or pretreated with salinomycin at concentration of 0.01, 0.1 and 1 μM, respectively. These cells were subjected to PEDV infection for 1 h with or without salinomycin treatment. After extensive washing using PBS, cells were further cultured for 24 h with the medium containing salinomycin or blank medium. Viral yields were then titrated by (a) western blot, (b) plaque assays and (d) indirect immunofluorescence assay (IFA). Green: PEDV N protein; Blue: DAPI; Scale bar, 20 μm. (c) Statistical of plaque assays results. The assays were performed in duplicate and data present as means ± SD. * p < 0.05, ** p < 0.01.