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Inhibition of PEDV replication by salinomycin is independent of reactive oxidative species (ROS) generation. Vero cells subjected to pretreatment with NAC (5 mM) or salinomycin(1 μM) for 1 h, were infected with PEDV along with NAC or salinomycin. At 24 hpi cellular ROS were detected using H2DCFDA (5 μM, 30 min). Values represent three independent experiments. Significant differences compared to untreated infected cells are indicated by *, (* p < 0.05), ns, no significance.
