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. 2021 Mar 25;10(4):628. doi: 10.3390/plants10040628

Figure 2.

Figure 2

A schematic view of genome editing by zinc finger nuclease (ZFN) and transcriptional activator-like effector nuclease (TALEN) in tef. A desired gene is selected from tef and integrated with ZFN and TALEN and then transferred to a cell through a vector, which will then introduce a break into the double-stranded DNA and integrate the gene of interest into the host genome. Transformed cells are used to regenerate to whole plants. (Note: the pictures used in this figure were either taken in the author’s labs or drawn using ChemBioDraw software).