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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Allergy. 2020 Mar 10;75(8):2005–2019. doi: 10.1111/all.14241

FIG 3. NLRP3 KO increases RV-induced innate cytokine expression, type 2 immune responses and mucus metaplasia in 6-day old wild type mice.

FIG 3.

Six day-old wild type C57BL/6 and NLRP3−/− mice were inoculated with sham or RV. A, B. One day after infection, whole lungs were homogenized in lysis buffer and subjected to Western blot. Anti-mouse-IL-1β recognizes pro-IL-1β and its bioactive form IL-1β. Anti-mouse-caspase-1 detects both caspase-1 and its cleaved form, caspase-1 p12. Group mean relative expression levels were normalized to β-actin. (N=3, mean±SEM, *different from wild type RV, p<0.05, one-way ANOVA.) C. mRNA expression of Il1b and Nlrp3 in wild type and NLRP3−/− mice. (N=4, mean±SEM, *different from WT sham, †different from WT RV, p<0.05, one-way ANOVA). D and E. PAS staining and Muc5ac immunofluorescence were examined 21 d post-infection (bar=50 μm). Whole-lung mRNA and protein expression were examined one or seven days post-infection. F-G Il33, Il1b, Nlrp3, Ifng, Tnf, Cxcl1 and Cxcl10 mRNA and IL-33 protein were examined one day post-infection; Il25, Il5, Il13, Muc5ac and Gob5 mRNA and IL-25 expression were examined 7 days post-infection (n =4, mean±SEM, *different from WT sham, †different from WT RV, p<0.05, one-way ANOVA). I. RV positive-strand RNA was assessed 24 h and 48 h after infection, and presented as viral copy number in total lung. (N =3–4, mean±SEM, *different from sham, †different from RV, p<0.05, one-way ANOVA).