Figure 2.

Characterization and neutralization of D1 on platelet aggregation. Neutralization of disintegrin-induced platelet aggregation inhibition by anti-disintegrin using human whole blood. (A) Illustrative aggregation curves recorded by the Chronolog aggregometer in whole blood. Chronolog aggregometer was used to measure ADP-induced platelet aggregation by impedance. (B) Neutralization of anti-platelet aggregation activity of disintegrin isolated from C. atrox by anti-disintegrin. A total of 10 μL of disintegrin/anti-disintegrin mixture was added to whole blood and incubated 2 min at 37 °C prior to adding 10 μM of ADP. The error bars represent the standard deviation from two independent experiments with n = 3. * Indicates significant differences between the PBS control and the disintegrin control (p < 0.05).