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. 2021 Mar 31;13(4):254. doi: 10.3390/toxins13040254

Figure 5.

Figure 5

Characterization of SVMP proteolytic activity and neutralization using anti-disintegrin. (A) Gelatinase activity of different C. o. helleri venom fractions. Twenty microliters of each fraction were placed on the gelatin film as described in the Materials and Methods. The proteolytic activity was measured by the clearing of a 10 mm spot on the film. Determination of SVMPs and SVSPs using inhibitors. Fractions 1, 7 and 8 were first incubated with inhibitors, 2 mM EDTA or 2 mM PMSF for 30 min, and 20 µL of sample were applied onto the gelatin film. (B) Neutralization of gelatinase activity of SVMPs using anti-disintegrin. Fractions 7 and 8 were first incubated with anti-disintegrin for 30 min and then 20 µL of each fraction were applied on the gelatin film and the proteolytic activity was measured. Crude venom and anti-disintegrin (Ab) were used as positive and negative controls, respectively.